the gel. The analytical gel was run with a Pippin Pulse using the 10-48kb pre-set protocol and run for 15 hours. 0.75% SeqKem Gold agarose from Lonza was used. 4 kb 5 kb 8 kb 6 kb 10 kb None (Control DNA) 6 kb 7 kb 8 kb 10 kb High-Pass threshold setting 29 kb 36 kb Marker S1 At the end of a run, marker peaks will be detected in designated ...Sep 26, 2023 · SDS-GHS: Reagent Replacement Kit for Dye Free Pippin Prep Cassettes (RIP2010, RIK2010, RIK 1510) SDS-GHS: Pippin Prep Cassettes with Ethidium Homodimer, Reagent Replacement Kit (REK2010) SDS-GHS: 10X KBB Electrophoresis Buffer (KBB1001) SDS-GHS 0.5X KBB buffer (PN190039) SDS-GHS: DNA Control Kits for Pippin Prep and BluePippin. Product Type. Agarose Gel Cassette. Separation Range. 100 to 600 bp. Quantity. 10 Pack. Provide Content Correction. For size selections between 100bp and 600bp. Sage Science™ BluePippin™ 2% Agarose Gel Cassettes are commonly used for Illumina paired-end library construction, Ion Torrent template prep, and ChIP applications.Dec 1, 2020 · Molecular characterization is a key step in the risk assessment of genetically modified organisms (GMOs) for regulatory approval. Herein, we describe a method for analyzing copy number, insertion loci, and flanking sequences through whole-genome sequencing (WGS) and bioinformatics. Comprehensive molecular characterization of G2-6 transgenic rice was performed using this pipeline. The results ... Internal standard sequences used for PippinPrep and BluePippin cassettes: 1.5% DF marker R2 and marker K 2%DF marker V1 and marker L 3% DF marker Q3 and marker PBlue Cross Bromsgrove rehoming centre, Bromsgrove. 11,320 likes · 141 talking about this · 986 were here. The Bromsgrove centre opened in 1986 and is now one of our largest centres. Located in...Sage Science Blue Pippin - Gel-based size-selection of fragments from 100bp to >30kb (depending on cassette and assay). Life Technologies Qubit 3.0 Fluorometer - Fluorescence-based quantitation using intercalating dye specific for DNA, RNA, or protein (so no contamination is included). Only 1μl of sample is required.We’ve taken the pulsed-field power supply out of the BluePippin instrument to provide researchers an affordable tool to resolve DNA out to 100 kb and beyond. The Pippin Pulse features a simple software interface to provide users full flexibility to develop their own field-inversion protocols. The operating range is 25-175V, and up to 200 mA ...BluePippin Operations Manual 460013 Rev D 1-1 1 Introduction Thank you for purchasing the BluePippin from Sage Science. The BluePippin is anThis was followed by phenol-chloroform purification and size selection to a minimum of 30 kb using a Blue Pippin. The resulting product was concentrated using ampure beads before library preparation was performed using a Rapid Barcoding Sequencing Kit (SQK-RBK001) modified through exclusion of LLB beads.Find the nearest stores. Search Address. Use current location. Seiko Prospex remains a standard for high-intensity timekeeping excellence. From the introduction of its first diver’s watch in 1965, Seiko has been a leader in undersea precision and durability, spearheading innovations in both technology and design.Sequencing of samples was performed using MiSeq Reagent Kit v3 (600 cycle; 2–300 bp) (Illumina, San Diego, CA, USA). For Long-read WGS, the library preparation and size selection were performed using PacBio Template Preparation 1.0 kit (Pacific Biosciences, Menlo Park, CA, USA) and Blue Pippin (Sage Sciences, Beverly, …Blue Pippin: Automated DNA Size Selection. Collect narrow and even fragment distributions; Flexibility to collect wide ranges of fragments with minimal effort; Pulsed-field electrophoresis for resolving and collecting high molecular weight DNA ...library was size-selected using a Blue Pippin (Sage Science), retaining only those fragments 300 bp ± 50 bp in length. This size-selection range was chosen by performing an in silico diges-tion of the published prairie rattlesnake, C. viridis viridis, genome [43], with the cut-site sequences of PstI (CTGCA^G) and MspI (C^CGG) using the program ...Services - BluePippin (SageScience) • BluePippin Cassette kits • Online Submission Request • Physical Submission of Samples. The BluePippin (jpg) has the capabilities of the Pippin Prep, plus pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer with no ...Merry And Pippin. His eyes are so blue ahh!! Save. Save. More like this. a close up of a person with blue eyes and curly hair wearing a black coat. This much ...The genome assembly of P. citrichinaensis genome (CBS 129764) was generated by the JGI using the PacBio (>10kb with Blue Pippin Size Selection) long-read sequencing technology. Long-read sequencing data was assembled using Flye 2.3.6 and the genome assembly was annotated using the JGI Annotation pipeline (Grigoriev et al. 2014).As a new type of fish, GF-L had a high fertilization rate (91.62%) and a hatching rate (82.67%). GF-L presented very beautiful phenotypes, such as twin tails, blue eyes and a white body accompanied by red spots. These phenotypes were very different from any other GF phenotype, showed that the GF-L lineage had great potential in the ornamental ...Building on the success of the Pippin Prep and Blue Pippin size selection technologies, the PippinHT provides the user with the ability to size select up to 24 samples. Sample Flexibility Like the Blue Pippin, the PippinHT has pulsed field capabilities, thus enabling the size selection of DNA fragments intended for use in both short-read ...Individual libraries were normalized to 10 nM and pooled. The library pool was purified with the QIAquick PCR Purification Kit (Qiagen AB, Sweden) according to instructions. Automated size selection was performed using the Blue Pippin (Sage Science, Beverly, MA, USA), with a range of 135 to 175 bp to select the ~152 bp fragments.from BioRad and Pippin Pulse System from Sage Science). Figure 2. Low DNA Input Sample and SMRTbell Library Preparation Workflow. To sequence a single low DNA input sample, ≥400 ng of input gDNA is required for the Sequel II System and the target DNA shear size distribution is 12 kb - 20 kb using the Megaruptor system. The library preparationWhen it comes to choosing a web host, there are many options available. One of the most popular choices is Blue Hosting. But how does it compare to other web hosts? In this article, we will explore the features and benefits of Blue Hosting ...Make dark blue paint by mixing blue and black paint together. This takes only a few moments to accomplish. You need blue paint, black paint, white paint, a container and a mixing stick.lected on a Sage Science Blue Pippin 1.5% cassette (Sage Science, Beverly, USA) to remove DNA molecules <600bp, and amplification-free, paired end libraries constructed usingThe published protocol 19 was used with minor modifications: size selection was performed using a Blue Pippin (Sage Science). In some instances we used rnh201-RED instead of rnh201::kan (see results).This video provides an overview of the basic types of DNA size selection protocols on the Pippin systems, and how they are programmed in the software interface.Fragments smaller than 20 kbp were removed using the Blue Pippin Size selection system (Sage Science, Beverly, MA, USA) and the constructed libraries were validated using Agilent 2100 Bioanalyzer ...Pippin Hill Farm is a unique culinary vineyard among the growing number of fine ... Blue Ridge Parkway Motorcycle Riding Blue Ridge Parkway Hiking Along The ...Jan 1, 2015 · I've found that if I use 3 serial 80% washes, my DNA is retained and I don't have salt problems in the Blue Pippin. ( N.B. I deliberately leave a little excess behind in the first 2 washes to ... The authors report on a high quality genome assembly of the model plant species Arabidopsis include two telomere-to-telomere assemblies (Chr 3 and 5). PacBio's HiFi, Oxford Nanopore ultra-long reads and Hi-C (Illumina) were used. BluePippin was used to size select large fragments from genomic extracts and for Hi-FI library construction.The end-repaired fragments were size-selected using the Blue Pippin Size-Selection System (Sage Science, MA, USA), and damage-repaired using the SMRTbell Damage Repair Kit (PacBio).Blue Cross is a registered charity in England and Wales (224392) and in Scotland (SC040154). A company limited by guarantee. Registered company in England and Wales under company number 00363197. Registered address Shilton Road, Burford, Oxfordshire, OX18 4PF.The Blue Pippin is a powerful automated tool to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular Next Generation Sequencing platforms. Features : Saves time and effort compared to manual gel purification; Sample-to-sample reproducibility streamlines researchPippin Prep DNA Size Selection System Selects 100 bp - 1.5 kb. SageELF Whole Sample Fractionation System DNA (up to 40 kb) or Proteins (up to 500 kDa). Pippin Pulse Electrophoresis Power Supply Resolves DNA up to 400kb on a midi gel box. Applications. Transposase Enzyme Linked Long-Read Sequencing ...Upgrading Pippin Instrument Software 1. Insert the USB drive with the upgrade folder into the USB port on the front panel of the BluePippin instrument. 2. From the Main Tab, press the "INFO" button. This will launch the information window. 3. From the Information Window, press "SOFTWARE UPGRADE", press"OK" at the warning prompt.Both approaches produced similar total yields of high-quality reads (roughly 3.5 gigabases, as shown by the point at which each line in Fig. 1C crosses the y-axis) but the yield of reads greater than 20KB was clearly higher when using the Blue Pippin, as shown by the red line in Figure 1C being higher than the blue line at a value of 20KB on ...This video covers the steps and processes for setting up a Pippin DNA size selection system.Art Prints · Greeting Cards · Commission Paintings · Custom Digital Creation · Short Video Editing & Creation · Family & Fun Focused!The bacterial wilt of peanut (Arachis hypogaea L.) caused by Ralstonia solanacearum is a devastating soil-borne disease that seriously restricted the world peanut production. However, the molecular mechanism of R. solanacearum-peanut interaction remains largely unknown. We found that R. solanacearum HA4-1 and PeaFJ1 isolated from peanut plants showed different pathogenicity by inoculating ...For safety's sake, keep your kids' bathing suits as bright as possible. When you shop for a swimsuit for your kid, don’t buy them a blue one. The simple reason: The color can make it difficult, even impossible, to see them underwater. Accor...The DNA was sheared to approximately 20kb, size separated using a Blue Pippin using the PAC-30 KB cassette (Sage Science). Libraries were prepared for PacBio sequencing using the large insert library protocol and Illumina sequencing using the TruSeq PCR-free kit per manufacturer recommendations. Long read sequencing was conducted on the Pacific ...American Robin is a US native and widely distributed songbird in Maine that lays unusual, bright blue eggs. Approximately 10 to 11 inches (25.5 - 28 cm) long bird spends winters in this state. It is quite a territorial species that enjoys hopping equally throughout the lawn and city parks.Nov 5, 2020 · 最主要的是普通的切胶回收只能回收中小片段，不能回收三代平台所需的大片段。. 因此手工切胶回收正在被其他更安全简便的方法替代。. 其次是磁珠分选，磁珠分选在二代测序中应用比较广泛。. 原理是磁珠会优先抓取较大的核酸片段，在分选过程中，通过 ... The High Pass Plus cassette has a stocky separation column, so DNA has a shorter distance to travel. The wider column and increased taper provide higher resolution for a cleaner and more accurate size cut-off. The larger sample wells now allow a maximum load of 10ug (100% up from standard Blue Pippin cassettes).Specifications. Concentration. 0.75%. Product Type. Agarose Gel Cassette. Quantity. 10 Pack. Provide Content Correction. Sage Science™ BluePippin™ Dye Free 0.75% Agarose Gel Cassettes are disposable and pre-cast cassettes with agarose and running buffer.BluePippin front panel USB Port Indicator Light: Indicator Light: Blue when instrument is Green when electrophoresis is ready for use (power is on, underway (protocol is running). and …The BluePippin is a preparative electrophoresis platform that separates and extracts DNA or protein from agarose. Using pre-cast gel cassettes, biomolecules are automatically collected in buffer according to software-input target ranges. The BluePippin also features pulsed-field electrophoresis power which enable accurate collection of kb-sized DNA. Cassettes are available for DNA size ...For each Nanopore library, the gDNA was size-selected (10-50 kb) with a Blue Pippin system (Sage Science, USA) and processed using the Ligation Sequencing 1D kit (SQKLSK109, Oxford Nanopore Technologies, UK) according to the manufacturer's instructions. Library construction and sequencing were done by the GridION X5/PromethION sequencer ...BluePippin DNA size selection system. The Sage Science BluePippin system is an automated DNA size selection tool for NGS applications, plus the capability of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA up to 50 kb. For long-read sequencing applications, high-pass filtering allows users to collect all ...Cassettes are disposable and pre-cast with agarose and running buffer. Samples are not optically detected.Blotting kits include all the components required to transfer protein, DNA, or RNA samples onto membranes for staining. The materials are grouped specifically for the method and the tissue specimen being implemented, allowing the entire procedure to be completed more rapidly. Using the ...For each isoform, blocks in blue represent exons, and lines between blocks represent introns. Red triangles indicate the position of primers used for RT-PCR. b RT-PCR validation of the differential intron retention level of PB.13680. cDNA from 5 h, 15 h and 30 h germinating seeds and leaf samples were used for RT-PCR. The gel bands show DNA ...Higher molecular weight fragments require a dedicated lane to run a calibration marker. Run times are as little as 30 minutes. To resolve very large DNA fragments, pulsed-field runs may last several hours. View the Blue Pippin cassette reference chart to explore the DNA size selection options and run times.The Blue Pippin applies pulsed-field electrophoresis technology to size select DNA for downstream applications (i.e. library preparation). This machine is extremely beneficial for high molecular weight applications due to its high pass filter that allows you to collect all fragments above a specified threshold.Library preparation and sequencing. We prepared three pooled sequencing libraries for the 438 samples following the 3RADseq protocol as described by Graham et al. (), with one modification: we size-selected for 650-850-bp fragments on a Blue Pippin (Sage Science).The concentration of each index group was determined by using a Qubit, and the average fragment length was determined by using a ...Approximately 30 μg of genomic DNA was used to construct PacBio libraries by shearing into ~ 20 kb targeted size fragments with Blue Pippin (Sage Science, Beverly, MA, USA).Background Orius laevigatus, a minute pirate bug, is a highly effective beneficial predator of crop pests including aphids, spider mites and thrips in integrated pest management (IPM) programmes. No genomic information is currently available for O. laevigatus, as is the case for the majority of beneficial predators which feed on crop pests. In contrast, genomic information for crop pests is ...Sage Science develops products for the life sciences research markets. Our mission is to provide new systems to streamline and improve sample preparation workflows for DNA and protein analysis. In 2010-12, the company commercialized the Pippin Prep and BluePippin preparative electrophoresis systems for targeted size selection of biomolecules. The Pippin platform has since established itself as ...Pippin Polar Bear - KC Needlepoint. Pre-Order. Pippin Polar Bear. $ 110.00 ... Bunny Blue Background Canvas - KC Needlepoint. Pre-Order. Bunny Blue Background ...targeting fragments 280–375 bp in size with Blue Pippin (Sage Science), and PCR amplified with indexed primers. Finally, the single-ended fragments weresequenced on the Illumina NextSeq500 with 150 cycles in high output mode [59]. 2.3. De novo assembly readsRepaired DNAs were selected by a Blue Pippin system to construct SMRTbell DNA libraries with fragment sizes of >10 kb according to the manufacturer's specification (PacBio, Menlo Park, CA, United States). Library quality was determined using a Qubit 2.0 Fluorometer (Life Technologies, CA, United States), and the average fragment size ...Shop Sea Women's Pippin Smocked Patchwork-printed Cotton Maxi Apron Dress In Blue Multi from 600+ stores, starting at $425. Similar ones also available.The Genome in a Bottle Consortium, hosted by the National Institute of Standards and Technology (NIST) is creating reference materials and data for human genome sequencing, as well as methods for ...BluePippin™ DNA Size Selection Instrument. Client: Sage Science. Tags: DNA Extraction & Purification, Instrumentation Life Sciences, NGS. The BluePippin is a preparative …In the first of the six runs, involving 13 samples, we used a Blue Pippin instrument to size-select DNA amplicons before sequencing. This was done to separate microbiome from host mitochondrial 16S rRNA gene sequences. This method proved untenable due to Blue Pippin performance inconsistencies and we did not apply …Sage Science, Inc. announced this month, the introduction of the BluePippin, an instrument that automates the size selection and retrieval of DNA for molecular biology research. The BluePippin expands Sage's Pippin line of preparative electrophoresis products by providing the capability to collect larger, kilobase-sized, DNA fragments. The Pippin platform is typically used to construct ...In the first of the six runs, involving 13 samples, we used a Blue Pippin instrument to size-select DNA amplicons before sequencing. This was done to separate microbiome from host mitochondrial 16S rRNA gene sequences. This method proved untenable due to Blue Pippin performance inconsistencies and we did not apply …Then the optimized cycle number was used to generate double-stranded cDNA, followed by size selection using the Blue Pippin TM Size-Selection System to generate three libraries (1-2 kb, 2-3 kb ...Blue Pippin回收的片段范围：50bp－50kp，样品通量：1－5个样品，回收效率：50－80％。Pippin Prep系统包括一次性的5泳道预制胶电泳槽，一个带有DNA荧光检测光路单元(非紫外)的电泳平台。 运行中，软件通过实时光路检测，和参考泳道中的DNA ladder进行比对，确定已经 ...Blue Pippin回收的片段范围：50bp－50kp，样品通量：1－5个样品，回收效率：50－80％。Pippin Prep系统包括一次性的5泳道预制胶电泳槽，一个带有DNA荧光检测光路单元(非紫外)的电泳平台。 运行中，软件通过实时光路检测，和参考泳道中的DNA ladder进行比对，确定已经 ...Internal standard sequences used for PippinPrep and BluePippin cassettes: 1.5% DF marker R2 and marker K 2%DF marker V1 and marker L 3% DF marker Q3 and marker PProduct Type. Agarose Gel Cassette. Separation Range. 100 to 600 bp. Quantity. 10 Pack. Provide Content Correction. For size selections between 100bp and 600bp. Sage Science™ BluePippin™ 2% Agarose Gel Cassettes are commonly used for Illumina paired-end library construction, Ion Torrent template prep, and ChIP applications.library was size-selected using a Blue Pippin (Sage Science), retaining only those fragments 300 bp ± 50 bp in length. This size-selection range was chosen by performing an in silico diges-tion of the published prairie rattlesnake, C. viridis viridis, genome [43], with the cut-site sequences of PstI (CTGCA^G) and MspI (C^CGG) using the program ...The size range of our cassettes can be found in our cassette kit reference charts for the Pippin Prep or the Blue Pippin. Most cassettes can accept up to 5 µg DNA per lane, but 1.5% cassettes can only be loaded with up to 2 µg DNA per lane. I ran out of marker. Can I use marker from a different instrument?Pippin. Sage Science 為專業的片段分離系統廠商，一直以來 Pippin Prep (100bp - 1.5kb)表現出卓越的分離結果，之後的Blue Pippin (100bp - 50kb) 更夾帶著Pulsed-field技術的高分辨率及回收率，令Sage Science 至今仍站穩全世界市占率最高的片段分離系統供應商，以及Blue Pippin ...The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. ...Men's Triple Crown Open Bottom Piped Adult Baseball Pants. 382. 50+ bought in past month. Save 5%. $1831. Typical: $19.31. Lowest price in 30 days. FREE delivery Sep 27 - 28.The Blue Pippin facilitates library construction for most popular NGS platforms, and is recommended by Pacific Biosciences, Illumina and Ion Torrent for certain workflows. Utilizing pulsed-field electrophoresis it is an ideal solution for resolving and collecting high molecular weight DNA up to 50kb. The platform features the ability to collect ...cDNA-Libraries für Next-Gen Sequencing-Applikationen. Die automatische Größenselektion mit den Pippin Systemen bietet viele Vorteile: High Quality Libraries – ...The Blue Pippin applies pulsed-field electrophoresis technology to size select DNA for downstream applications (i.e. library preparation). This machine is extremely beneficial for high molecular weight applications due to its high pass filter that allows you to collect all fragments above a specified threshold.Note, however, that the CHEF Mapper and Pippin Pulse systems require at least 50 ng of DNA for analy, Pippin Hills is a boutique venue and winery located just outside of Charlottesville, Virginia, at the foothills of t, Blue Pippin Usage Form Date: Name: PI Name: Account #: Billing Contact: Billing Address: Cassettes Used (enter, The Blue Pippin is preparative electrophoresis sys, In our Iso-Seq analysis, we detected a total of 27,860 tran, The Blue Pippin is a powerful automated tool to collect DNA fragments up to 50 kb or si, This video provides an overview of the basic types of DNA size selection, , Sage Science Blue Pippin - Gel-based size-selection of fragments f, Run times are as little as 30 minutes. To resolve very large DNA fra, The BluePippin allows researchers to easily size select and col, The Paenibacillus sp. strain B-LR circular genome is 6,694,76, Are you looking to get the most out of your 2007 Honda Civic? T, Both approaches produced similar total yields of high-qua, Cassette Kit Product Number Cassette Type Software DNA Contro, Specifications. Concentration. 0.75%. Product Type. Agarose Ge, Size selection (> 20 kb) was performed using 15 μg of genomic DNA a, Blue Pippin Covaris M220 UV-Vis | Tecan Plate Reade.